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黄芩是一种重要的药用植物，其根部富含黄酮类化合物，具有多种药理活性。为探究不同花色黄芩种质间黄酮类化合物的差异积累机制，本研究选取白花、粉花、蓝紫花和紫花黄芩种质，采用代谢组学和转录组学联合分析技术，鉴定差异代谢物和差异表达基因。通过代谢组学分析，共检测到1 823种代谢物，其中黄酮类化合物576种，从中筛选出35种差异显著的黄酮代谢物，如紫花黄芩中的柚皮素查尔酮、矢车菊素和白花黄芩中的山柰酚等。通过转录组测序分析，共鉴定出5 331个差异表达基因，其中33个与黄酮合成途径相关，包括CHS、ANS、FLS和AOMT等关键酶基因；此外，预测到MYB、ERF和bZIP等转录因子家族可能参与调控黄酮合成相关基因的表达。随机选取8个黄酮合成相关差异表达基因进行qRT-PCR验证，结果与转录组数据趋势一致。本研究揭示了不同花色黄芩种质黄酮类化合物差异积累的分子机制，可为优良黄芩种质选育和次生代谢调控提供理论依据。
Abstract：Scutellaria baicalensis, a vital medicinal plant, contains flavonoids in roots with diverse pharmacological activities. To investigate the differential accumulation mechanism of flavonoids among different germplasms, the white-flowered, pink-flowered, blue-purple-flowered and purple-flowered S. baicalensis germplasms were selected for the integrated metabolomics and transcriptomics analyses to identify differential metabolites and genes. Through metabolomic analysis, 1 823 metabolites were detected including 576 flavonoids, among which, 35 significantly different flavonoid metabolites were identified, such as naringin chalcone and rutin in purple-flowered S. baicalensis, and kaempferol in white-flowered S. baicalensis. Through transcriptomic sequencing, 5 331 differentially expressed genes(DEGs) were identified, including 33 genes involved in flavonoid biosynthesis pathways like CHS, ANS, FLS and AOMT. Additionally, MYB, ERF and bZIP transcription factor families were predicted to regulate flavonoid synthesis-related gene expression. Eight DEGs related to flavonoid biosynthesis were randomly selected for the qRT-PCR validation, and the results corroborated the transcriptomic data. This study elucidated the molecular mechanisms of flavonoid accumulation differences among different flower color S. baicalensis germplasms, which could provide theoretical foundations for germplasm improvement and secondary metabolite regulation.
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基本信息:
DOI：10.14083/j.issn.1001-4942.2026.02.001
中图分类号:S567.239
引用信息:
[1]王庭硕,郭凤丹,赵一伍,等.基于代谢组学及转录组学研究不同种质黄芩黄酮类成分差异积累的转录调控机制[J].山东农业科学,2026,58(02):1-12.DOI:10.14083/j.issn.1001-4942.2026.02.001.
基金信息:
山东省科技型中小企业创新能力提升工程项目(2022TSGC1059,2023TSGC0444); 中央本级重大增减支项目(2060302); 山东省中医药高层次人才培育项目(2023143); 全国中药特色技术传承人才培训项目(202396)
2025-08-04
2025
2026-02-05
2026
2
2026-02-09
2026-02-09
2026-02-09