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小G蛋白Rab是植物中重要的分子开关，参与调节植物生长发育进程，响应外界生物和非生物胁迫反应。本研究以番茄为材料，克隆获得番茄内源小G蛋白基因SlRab5b的cDNA序列，并对其进行生物信息学分析和表达模式分析。结果表明，SlRab5b的开放阅读框(ORF)大小为603 bp,编码200个氨基酸，SlRAB5b蛋白是一个无跨膜结构、无信号肽的蛋白。进化树分析表明，SlRAB5b为RABF家族成员，与拟南芥AtRABF1和AtRABF2b蛋白同源性分别高达86.70%和59.52%,且具有和其他植物中RABF蛋白相似的保守序列。供试4个番茄品种中，除918外不同组织中SlRab5b基因相对表达量表现为茎>新叶>老叶>根。茉莉酸甲酯处理48 h或水杨酸处理6 h,SlRab5b基因的相对表达量较对照分别显著增加1 745.8%、548.3%。接种晚疫菌后SlRab5b基因的相对表达量也呈上调趋势，接菌后48 h,番茄品种YS-7、918中SlRab5b基因相对表达量分别较对照显著增加432.7%、426.1%。由此可知，SlRab5b基因的表达在番茄不同组织中存在差异，同时还能被晚疫菌侵染和茉莉酸甲酯、水杨酸处理所诱导。
Abstract：Small G protein Rab is an important molecular switch in the plants, which participates in regulating plant growth and development processes, and responding to external biotic and abiotic stresses. In this study, the cDNA sequence of tomato endogenous small G protein gene SlRab5b was cloned using tomato plants as materials, and its bioinformatics analysis and expression pattern analysis were conducted. The results showed that the open reading frame(ORF) of SlRab5b was 603 bp, encoding 200 amino acids. It was a protein without transmembrane structure and signal peptide. Phylogenetic tree analysis showed that SlRAB5b was a member of the RABF family with the conserved sequence similar to RABF proteins in other plants, and had 86.70% and 59.52% homology with Arabidopsis AtRABF1 and AtRABF2b proteins. The relative expression level of SlRab5b gene in different tissues of tomato showed stem > new leaf > old leaf > root in the tested varieties except 918. After 48 h of methyl jasmonate(MeJA) treatment and 6 h of salicylic acid(SA) treatment, the relative expression level of SlRab5b gene significantly increased by 1 745.8% and 548.3%, compared with control, respectively. The relative expression level of SlRab5b gene was also up-regulated after inoculation with Phytophthora infestans, which was significantly increased in tomato varieties YS-7 and 918 by 432.7% and 426.1%, respectively, compared with control 48 h after inoculation. It could be seen that the expression of SlRab5b gene was different in different tissues of tomato, and could be induced by the infection of pathogenic bacteria of late blight and the treatment of MeJA and SA.
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基本信息:
DOI：10.14083/j.issn.1001-4942.2026.04.002
中图分类号:S641.2;Q943.2
引用信息:
[1]伊海佼,孙学桃,赵玉平,等.番茄SlRab5b基因的克隆与表达模式分析[J].山东农业科学,2026,58(04):8-15.DOI:10.14083/j.issn.1001-4942.2026.04.002.
基金信息:
内蒙古自治区自然科学基金项目(2023MS03018);内蒙古自治区自然科学基金重点项目(2022ZD10)
2024-12-11
2024
2026-04-05
2026-04-10
2026
2
2026-04-15
2026-04-15
2026-04-15