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HLS1为调控植物顶端弯钩的关键基因，同时参与植物发育的多个过程。CsHLS1通过调控黄瓜叶片和叶柄角大小影响黄瓜的株型。然而，CsHLS1在黄瓜中是否有同源基因及其潜在的生物学功能均待于解析。本研究通过构建CsHLS1的系统发育树挖掘到黄瓜中与其高度同源的一个蛋白，将其命名为CsHLS2,继而进一步克隆了黄瓜CsHLS2并研究其分子特征和表达特性。结果表明，CsHLS2基因全长2 152 bp,编码278个氨基酸；编码蛋白分子量为32.25 kDa,理论等电点为9.03,不稳定系数为39.24,脂肪系数为84.17,总平均亲水性系数为-0.415,不含信号肽和跨膜结构域，属于稳定蛋白；二级结构以无规卷曲(59.71%)与α-螺旋(26.26%)为主；系统发育以及蛋白保守结构域分析结果表明，CsHLS2与甜瓜CmHLS2的亲缘关系最近。对CsHLS2基因启动子进行克隆并分析其作用元件，结果显示该区域含有多个环境响应元件和激素响应元件。RT-qPCR结果表明，该基因在黄瓜的子房和叶柄基部高表达。研究结果可为解析CsHLS2在黄瓜生长发育中的功能提供参考。
Abstract：HLS1 is a key gene regulating plant apical hook formation and is also involved in multiple plant developmental processes. CsHLS1 modulates plant architecture in cucumber by regulating the sizes of leaf and petiole angles. However, whether CsHLS1 has homologs in cucumber and their potential biological functions remained to be elucidated. In this study, a protein highly homologous to CsHLS1 was identified in cucumber by constructing a phylogenetic tree of CsHLS1, which was designated as CsHLS2. Furthermore, CsHLS2 from cucumber was cloned and its molecular characteristics and expression profiles were investigated. The results showed that the full-length sequence of CsHLS2 was 2 158 bp, encoding 278 amino acids. The CsHLS2 protein had the molecular weight of 32.25, the theoretical isoelectric point of 9.03, the instability index of 9.24, the aliphatic index of 84.17, the grand average of hydropathicity of-0.415, and had no signal peptide or transmembrane domain, indicating that it was a stable protein. The secondary structure of CsHLS2 was mainly composed of random coils(59.71%) and α-helices(26.26%). Phylogenetic analysis and conserved domain analysis revealed that CsHLS2 in cucumber had the closest genetic relationship with CmHLS2 in melon(Cucumis melo). The promoter region of CsHLS2 was cloned and analyzed for its action elements, and the result demonstrated that this region contained multiple environmental and hormone-responsive elements. Quantitative real-time PCR(RT-qPCR) analysis indicated that CsHLS2 was highly expressed in cucumber ovaries and petiole bases. These findings could provide a foundation for further elucidating the functional roles of CsHLS2 in cucumber growth and development.
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基本信息:
DOI：10.14083/j.issn.1001-4942.2026.01.003
中图分类号:S642.2
引用信息:
[1]方凯,杜亚琳,郝宁,等.黄瓜CsHLS2基因的克隆与表达分析[J].山东农业科学,2026,58(01):17-26.DOI:10.14083/j.issn.1001-4942.2026.01.003.
基金信息:
国家自然科学基金联合基金重点支持项目(U23A20209,U25A20691);国家自然科学基金面上项目(32372679,32372700); 湖南省自然科学基金项目(2023JJ10021)
2026-01-22
2026-01-22
2026-01-22